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Home > Product > ELISA Kit > Mouse Methylmalonyl-CoA Mutase, Mitochondrial, MMUT ELISA Kit

Mouse Methylmalonyl-CoA Mutase, Mitochondrial, MMUT ELISA Kit

Mouse Methylmalonyl-CoA Mutase, Mitochondrial, MMUT ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 12.01ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 12.01ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Methylmalonyl-CoA Mutase, Mitochondrial

Code

E1756Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

12.01ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.

UniProt accession

MASS(Da)

82,844

GeneID

17850

Synonyms

D230010K02Rik;Mcm;Methylmalonyl-CoA isomerase;Methylmalonyl-CoA mutase, mitochondrial;Mut

Gene names

Mmut

Research area

Signal Transduction

Target protein

MCM

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1756Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.557 2.481 2.419
2.404
1000ng/L 1.422 1.494 1.432
1.565
500ng/L 0.77 0.79 0.727
0.809
250ng/L 0.448 0.476 0.414
0.504
125ng/L 0.219 0.238 0.176
0.257
0ng/L 0.065 0.062 0
0.059

Precision

We measured random samples of E1756Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1083.32 36.51 3.37
Intra-Assay 2 18 821.86 39.07 4.75
Intra-Assay 3 18 365.44 21.58 5.91

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1756Mo

    $320.00

  • 96T

    E1756Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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