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Home > Product > ELISA Kit > Human Aromatase, ARO ELISA Kit

Human Aromatase, ARO ELISA Kit

Human Aromatase, ARO ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aromatase

Code

E3348Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed: 27702664, PubMed: 2848247). Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed: 20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed: 22773874). Also displays 2-hydroxylase activity toward estrone (PubMed: 22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed: 20385561, PubMed: 22773874).

UniProt accession

MASS(Da)

57,883

GeneID

1588

Synonyms

Aromatase;CYP19A1;CYPXIX;Cytochrome P450 19A1;Cytochrome P-450AROM;Estrogen Synthase

Gene names

CYP19A1

Research area

Metabolism

Target protein

CYP19A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3348Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.135 2.072 2.025
2.009
16ng/ml 1.523 1.482 1.435
1.441
8ng/ml 0.801 0.831 0.785
0.861
4ng/ml 0.447 0.49 0.444
0.534
2ng/ml 0.245 0.251 0.205
0.258
0ng/ml 0.034 0.047 0
0.059

Precision

We measured random samples of E3348Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 14 0.59 4.2
Intra-Assay 2 18 5.2 0.29 5.7
Intra-Assay 3 18 6.8 0.38 5.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3348Hu

    $320.00

  • 96T

    E3348Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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