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Home > Product > ELISA Kit > Mouse Agouti-signaling Protein, ASIP ELISA Kit

Mouse Agouti-signaling Protein, ASIP ELISA Kit

Mouse Agouti-signaling Protein, ASIP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.72ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Agouti-signaling protein

Code

E1826Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.72ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.

UniProt accession

MASS(Da)

14,313

GeneID

50518

Synonyms

A;ago;Agouti coat color protein;Agouti switch protein;Agouti-signaling protein;As;ASIP;ASP

Gene names

Asip

Research area

Signal Transduction

Target protein

ASIP

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1826Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.206 2.232 2.176
2.258
1600ng/L 1.453 1.451 1.396
1.45
800ng/L 0.778 0.818 0.762
0.859
400ng/L 0.417 0.483 0.427
0.548
200ng/L 0.32 0.32 0.264
0.32
0ng/L 0.054 0.056 0
0.057

Precision

We measured random samples of E1826Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 562.68 36.35 6.46
Intra-Assay 2 18 2237.25 76.88 3.44
Intra-Assay 3 18 1127.35 54.92 4.87

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1826Mo

    $320.00

  • 96T

    E1826Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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