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Home > Product > ELISA Kit > Rat 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Gamma-1, PLCG1 ELISA Kit

Rat 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Gamma-1, PLCG1 ELISA Kit

Rat 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Gamma-1, PLCG1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.26ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 7.26ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1

Code

E1833Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.26ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed:7531435). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:7531435). Plays a role in actin reorganization and cell migration (By similarity).

UniProt accession

MASS(Da)

148,548

GeneID

25738

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-gamma-1;PLCG1;PLC-gamma-1;PPLCA

Gene names

Plcg1

Research area

Cardiovascular

Target protein

PLCG1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1833Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.357 2.201 2.162
2.044
800ng/L 1.541 1.568 1.529
1.595
400ng/L 1.039 1.004 0.965
0.969
200ng/L 0.721 0.725 0.687
0.729
100ng/L 0.441 0.467 0.429
0.494
0ng/L 0.028 0.039 0
0.05

Precision

We measured random samples of E1833Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 568.66 24.28 4.27
Intra-Assay 2 18 229.43 17.2 7.5
Intra-Assay 3 18 189.55 15.82 8.34

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E1833Ra

    $320.00

  • 96T

    E1833Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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