Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1865Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 1.834 | 1.913 | 1.877 |
| 1.992 | |||
| 6ng/mL | 0.992 | 0.947 | 0.911 |
| 0.902 | |||
| 3ng/mL | 0.546 | 0.57 | 0.533 |
| 0.593 | |||
| 1.5ng/mL | 0.271 | 0.237 | 0.201 |
| 0.203 | |||
| 0.75ng/mL | 0.159 | 0.133 | 0.097 |
| 0.107 | |||
| 0ng/mL | 0.041 | 0.036 | 0 |
| 0.032 |
Precision
We measured random samples of E1865Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 11.15 | 0.35 | 3.16 |
| Intra-Assay | 2 | 18 | 4.53 | 0.22 | 4.82 |
| Intra-Assay | 3 | 18 | 4.36 | 0.25 | 5.7 |
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