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Home > Product > ELISA Kit > Mouse B- and T-lymphocyte Attenuator, BTLA ELISA Kit

Mouse B- and T-lymphocyte Attenuator, BTLA ELISA Kit

Mouse B- and T-lymphocyte Attenuator, BTLA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

B- and T-lymphocyte attenuator

Code

E1866Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.12ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

B- and T-lymphocyte attenuator or BTLA (also known as cluster of differentiation 272 or CD272) is a protein that belongs to the CD28 immunoglobulin superfamily (IgSF) which is encoded by the BTLA gene located on the 3rd human chromosome. BTLA was first discovered in 2003 as an inhibitor of Th1 expansion and it became the 3rd member of the CD28 IgSF. However, its discovered ligand herpes virus entry mediator or HVEM (also known as tumour necrosis factor receptor superfamily member 14 or TNFRSF14) belongs to the tumor necrosis factor receptor superfamily (TNFRSF). This finding was surprising because until the discovery of HVEM it was believed that receptors and ligands always belong to the same family.

UniProt accession

MASS(Da)

34,337

GeneID

208154

Synonyms

A630002H24;B- and T-lymphocyte attenuator;B- and T-lymphocyte-associated protein;BTLA;CD antigen CD272

Gene names

Btla

Research area

Immunology

Target protein

BTLA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1866Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 2.115 2.046 2.014
1.976
24ng/ml 1.357 1.424 1.392
1.491
12ng/ml 0.652 0.646 0.614
0.639
6ng/ml 0.327 0.332 0.3
0.337
3ng/ml 0.155 0.145 0.113
0.135
0ng/ml 0.027 0.032 0
0.037

Precision

We measured random samples of E1866Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.64 0.51 4.4
Intra-Assay 2 18 35.84 1.62 4.53
Intra-Assay 3 18 19.4 0.97 4.99

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1866Mo

    $320.00

  • 96T

    E1866Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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