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Home > Product > ELISA Kit > Rat Apoptosis Regulator Bax, BAX ELISA Kit

Rat Apoptosis Regulator Bax, BAX ELISA Kit

Rat Apoptosis Regulator Bax, BAX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.13ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 10.13ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Apoptosis regulator BAX

Code

E1869Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.13ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

UniProt accession

MASS(Da)

21,351

GeneID

24887

Synonyms

Apoptosis regulator BAX;BAX

Gene names

Bax

Research area

Cancer

Target protein

BAX

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1869Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.171 2.203 2.157
2.236
2000ng/L 1.465 1.419 1.372
1.373
1000ng/L 0.734 0.831 0.785
0.929
500ng/L 0.502 0.509 0.463
0.517
250ng/L 0.252 0.242 0.195
0.231
0ng/L 0.051 0.047 0
0.042

Precision

We measured random samples of E1869Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 912.89 52.51 5.75
Intra-Assay 2 18 2849.93 115.7 4.06
Intra-Assay 3 18 760.46 50.3 6.61

Protocols

References (1)

Ferulic acid exerts a protective effect against cyclosporine-induced liver injury in rats via activation of the Nrf2/HO-1 signaling, suppression of oxidative stress, inflammatory response, and halting the apoptotic cell death

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  • Size

    Code

    Price

  • 48T

    E1869Ra

    $320.00

  • 96T

    E1869Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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