Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1881Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 1.82 | 1.919 | 1.869 |
| 2.018 | |||
| 1200ng/L | 1.058 | 1.093 | 1.043 |
| 1.127 | |||
| 600ng/L | 0.421 | 0.472 | 0.423 |
| 0.523 | |||
| 300ng/L | 0.253 | 0.271 | 0.222 |
| 0.29 | |||
| 150ng/L | 0.083 | 0.068 | 0.019 |
| 0.054 | |||
| 0ng/L | 0.059 | 0.049 | 0 |
| 0.04 |
Precision
We measured random samples of E1881Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1624.14 | 55.94 | 3.44 |
| Intra-Assay | 2 | 18 | 1384.67 | 68.48 | 4.95 |
| Intra-Assay | 3 | 18 | 838.7 | 40.33 | 4.81 |
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