Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1891Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/mL | 2.109 | 2.166 | 2.123 |
| 2.224 | |||
| 240ng/mL | 1.686 | 1.784 | 1.741 |
| 1.882 | |||
| 120ng/mL | 1.187 | 1.207 | 1.164 |
| 1.227 | |||
| 60ng/mL | 0.672 | 0.647 | 0.604 |
| 0.621 | |||
| 30ng/mL | 0.405 | 0.401 | 0.358 |
| 0.398 | |||
| 0ng/mL | 0.055 | 0.043 | 0 |
| 0.031 |
Precision
We measured random samples of E1891Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 241.75 | 15.87 | 6.56 |
| Intra-Assay | 2 | 18 | 85.76 | 4.64 | 5.41 |
| Intra-Assay | 3 | 18 | 38.18 | 2.22 | 5.81 |
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