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Home > Product > ELISA Kit > Rat Carboxypeptidase B, CPB1 ELISA Kit

Rat Carboxypeptidase B, CPB1 ELISA Kit

Rat Carboxypeptidase B, CPB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.12ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.12ng/mL

Detection range: 7-1500ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Carboxypeptidase B

Code

E1894Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.12ng/mL

Detection range

7-1500ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

UniProt accession

MASS(Da)

47,515

GeneID

24271

Synonyms

Carboxypeptidase B;Cpb;CPB1

Gene names

Cpb1

Research area

Signal Transduction

Target protein

CPB1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1894Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/mL 2.081 2.072 2.027
2.063
400ng/mL 1.837 1.839 1.794
1.841
200ng/mL 1.24 1.193 1.148
1.146
100ng/mL 0.668 0.666 0.621
0.665
50ng/mL 0.365 0.368 0.323
0.371
0ng/mL 0.06 0.045 0
0.03

Precision

We measured random samples of E1894Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 305.39 18.15 5.94
Intra-Assay 2 18 174.82 9.02 5.16
Intra-Assay 3 18 61.36 2.71 4.41

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1894Ra

    $320.00

  • 96T

    E1894Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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