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Home > Product > ELISA Kit > Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Human Alpha-methylacyl-CoA Recemase, AMACR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.16ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Alpha-Methylacyl-Coa Recemase

Code

E3387Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.16ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359).

UniProt accession

MASS(Da)

42,387

GeneID

23600

Synonyms

2-methylacyl-CoA racemase;Alpha-methylacyl-CoA racemase;AMACR

Gene names

AMACR

Research area

Others

Target protein

AMACR

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3387Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.436 2.436 2.389
2.436
1200ng/L 1.433 1.412 1.364
1.39
600ng/L 0.944 0.928 0.881
0.912
300ng/L 0.487 0.464 0.417
0.442
150ng/L 0.255 0.251 0.204
0.248
0ng/L 0.042 0.047 0
0.052

Precision

We measured random samples of E3387Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 545.4 30.62 5.6
Intra-Assay 2 18 1506.3 52.96 3.5
Intra-Assay 3 18 496.3 31.3 6.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3387Hu

    $320.00

  • 96T

    E3387Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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