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Home > Product > ELISA Kit > Rat Defensin-5, DEFA5 ELISA Kit

Rat Defensin-5, DEFA5 ELISA Kit

Rat Defensin-5, DEFA5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

defensin-5

Code

E1944Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/mL

Detection range

0.5-200ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Defensin, alpha 5 (DEFA5) also known as human alpha defensin 5 (HD5) is a protein that in humans is encoded by the DEFA5 gene. DEFA5 is expressed in the Paneth cells of the ileum. Defensins are a family of microbicidal and cytotoxic peptides (antimicrobial peptides; AMP) that are involved in host defense, and help to maintain homeostasis of intestinal microbiota. DEFA5 is the main AMP that controls the enteric microbiota composition by selective killing of bacterial pathogens while preserving commensals.

UniProt accession

MASS(Da)

10,242

GeneID

286995

Synonyms

Defa;DEFA5;Defensin 5;Enteric defensin;RD-5

Gene names

DEFA5

Research area

Microbiology

Target protein

DEFA5

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1944Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/mL 2.286 2.27 2.225
2.253
60ng/mL 1.332 1.401 1.356
1.469
30ng/mL 0.893 0.81 0.765
0.726
15ng/mL 0.42 0.432 0.387
0.444
7.5ng/mL 0.271 0.253 0.208
0.234
0ng/mL 0.051 0.045 0
0.038

Precision

We measured random samples of E1944Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 80.95 2.77 3.42
Intra-Assay 2 18 43.02 1.84 4.27
Intra-Assay 3 18 17.32 0.91 5.23

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1944Ra

    $320.00

  • 96T

    E1944Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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