Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1960Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/mL | 2.287 | 2.315 | 2.261 |
| 2.344 | |||
| 60ng/mL | 1.77 | 1.836 | 1.781 |
| 1.902 | |||
| 30ng/mL | 1.314 | 1.232 | 1.177 |
| 1.151 | |||
| 15ng/mL | 0.695 | 0.688 | 0.633 |
| 0.682 | |||
| 7.5ng/mL | 0.352 | 0.394 | 0.339 |
| 0.436 | |||
| 0ng/mL | 0.055 | 0.055 | 0 |
| 0.055 |
Precision
We measured random samples of E1960Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 14.69 | 0.92 | 6.23 |
| Intra-Assay | 2 | 18 | 25.04 | 1.6 | 6.41 |
| Intra-Assay | 3 | 18 | 46.19 | 1.95 | 4.23 |
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