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Home > Product > ELISA Kit > Rat G Protein-coupled Receptor Kinase 6, GRK6 ELISA Kit

Rat G Protein-coupled Receptor Kinase 6, GRK6 ELISA Kit

Rat G Protein-coupled Receptor Kinase 6, GRK6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.69ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.69ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

G protein-coupled receptor kinase 6

Code

E1969Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.69ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro) (By similarity).

UniProt accession

MASS(Da)

65,963

GeneID

59076

Synonyms

G protein-coupled receptor kinase 6;G protein-coupled receptor kinase GRK6;Gprk6;GRK6

Gene names

Grk6

Research area

Signal Transduction

Target protein

GRK6

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1969Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.286 2.311 2.279
2.337
1200ng/L 1.378 1.347 1.314
1.316
600ng/L 0.756 0.689 0.657
0.623
300ng/L 0.406 0.412 0.379
0.418
150ng/L 0.163 0.149 0.117
0.136
0ng/L 0.025 0.033 0
0.04

Precision

We measured random samples of E1969Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 993.9 64.63 6.5
Intra-Assay 2 18 1321.34 40.13 3.04
Intra-Assay 3 18 505.34 27.89 5.52

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1969Ra

    $320.00

  • 96T

    E1969Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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