Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1982Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.112 | 2.08 | 2.045 |
| 2.048 | |||
| 12ng/mL | 1.083 | 1.072 | 1.037 |
| 1.062 | |||
| 6ng/mL | 0.502 | 0.488 | 0.453 |
| 0.474 | |||
| 3ng/mL | 0.175 | 0.193 | 0.158 |
| 0.212 | |||
| 1.5ng/mL | 0.127 | 0.147 | 0.112 |
| 0.168 | |||
| 0ng/mL | 0.043 | 0.035 | 0 |
| 0.026 |
Precision
We measured random samples of E1982Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 6.07 | 0.2 | 3.37 |
| Intra-Assay | 2 | 18 | 18.25 | 0.56 | 3.04 |
| Intra-Assay | 3 | 18 | 9.8 | 0.38 | 3.87 |
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