Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1985Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.645 | 2.583 | 2.55 |
| 2.521 | |||
| 800ng/L | 1.596 | 1.563 | 1.531 |
| 1.53 | |||
| 400ng/L | 0.798 | 0.84 | 0.807 |
| 0.882 | |||
| 200ng/L | 0.378 | 0.408 | 0.375 |
| 0.437 | |||
| 100ng/L | 0.262 | 0.243 | 0.211 |
| 0.224 | |||
| 0ng/L | 0.034 | 0.032 | 0 |
| 0.031 |
Precision
We measured random samples of E1985Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 769.9 | 25.53 | 3.32 |
| Intra-Assay | 2 | 18 | 529.83 | 27.16 | 5.13 |
| Intra-Assay | 3 | 18 | 314.72 | 16.38 | 5.21 |
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