Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1986Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 2.19 | 2.206 | 2.144 |
| 2.222 | |||
| 6ng/mL | 1.536 | 1.452 | 1.39 |
| 1.369 | |||
| 3ng/mL | 0.803 | 0.875 | 0.813 |
| 0.948 | |||
| 1.5ng/mL | 0.454 | 0.498 | 0.435 |
| 0.542 | |||
| 0.75ng/mL | 0.305 | 0.286 | 0.223 |
| 0.266 | |||
| 0ng/mL | 0.058 | 0.062 | 0 |
| 0.066 |
Precision
We measured random samples of E1986Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.43 | 0.08 | 5.82 |
| Intra-Assay | 2 | 18 | 5.91 | 0.25 | 4.23 |
| Intra-Assay | 3 | 18 | 4.55 | 0.28 | 6.08 |
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