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Home > Product > ELISA Kit > Rat Glutathione Synthetase, GSS ELISA Kit

Rat Glutathione Synthetase, GSS ELISA Kit

Rat Glutathione Synthetase, GSS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Glutathione synthetase

Code

E1989Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Glutathione synthetase (GSS) (EC 6.3.2.3) is the second enzyme in the glutathione (GSH) biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Glutathione synthetase is also a potent antioxidant. It is found in many species including bacteria, yeast, mammals, and plants.

UniProt accession

MASS(Da)

52,345

GeneID

25458

Synonyms

Glutathione Synthase;Glutathione synthetase;GSH synthetase;GSH-S;GSS

Gene names

Gss

Research area

Signal Transduction

Target protein

GSS

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1989Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.146 2.161 2.107
2.176
6ng/mL 1.391 1.445 1.391
1.499
3ng/mL 0.815 0.811 0.758
0.807
1.5ng/mL 0.532 0.482 0.428
0.431
0.75ng/mL 0.285 0.251 0.197
0.216
0ng/mL 0.054 0.054 0
0.053

Precision

We measured random samples of E1989Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.25 0.34 4.14
Intra-Assay 2 18 3.27 0.19 5.75
Intra-Assay 3 18 1.15 0.05 4.23

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1989Ra

    $320.00

  • 96T

    E1989Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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