Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1995Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.053 | 2.112 | 2.06 |
| 2.171 | |||
| 1000ng/L | 1.468 | 1.48 | 1.429 |
| 1.493 | |||
| 500ng/L | 0.736 | 0.807 | 0.756 |
| 0.878 | |||
| 250ng/L | 0.44 | 0.439 | 0.388 |
| 0.439 | |||
| 125ng/L | 0.308 | 0.295 | 0.243 |
| 0.282 | |||
| 0ng/L | 0.058 | 0.052 | 0 |
| 0.045 |
Precision
We measured random samples of E1995Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1019.42 | 46.03 | 4.52 |
| Intra-Assay | 2 | 18 | 512.28 | 22.32 | 4.36 |
| Intra-Assay | 3 | 18 | 338.84 | 19.37 | 5.72 |
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