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Home > Product > ELISA Kit > Mouse Disintegrin and Metalloproteinase Domain-containing Protein 15, ADAM15 ELISA Kit

Mouse Disintegrin and Metalloproteinase Domain-containing Protein 15, ADAM15 ELISA Kit

Mouse Disintegrin and Metalloproteinase Domain-containing Protein 15, ADAM15 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Disintegrin and metalloproteinase domain-containing protein 15

Code

E2025Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.19ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration (By similarity). Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. Interactions with egg membrane could be mediated via binding between the disintegrin-like domain to one or more integrin receptors on the egg.

UniProt accession

MASS(Da)

92,664

GeneID

11490

Synonyms

AD56;ADAM 15;ADAM15;Disintegrin and metalloproteinase domain-containing protein 15;MDC15;MDC-15;Metalloprotease RGD disintegrin protein;Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15;metar;Metargidin

Gene names

Adam15

Research area

Signal Transduction

Target protein

ADAM15

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2025Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.612 2.496 2.467
2.381
600ng/L 1.58 1.478 1.448
1.376
300ng/L 0.903 0.879 0.85
0.856
150ng/L 0.528 0.5 0.471
0.472
75ng/L 0.236 0.238 0.209
0.241
0ng/L 0.027 0.03 0
0.032

Precision

We measured random samples of E2025Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 573.88 20.16 3.51
Intra-Assay 2 18 363.33 19.92 5.48
Intra-Assay 3 18 117.5 6.81 5.79

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2025Mo

    $320.00

  • 96T

    E2025Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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