Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2051Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/mL | 2.637 | 2.524 | 2.491 |
| 2.412 | |||
| 24ng/mL | 1.346 | 1.373 | 1.339 |
| 1.399 | |||
| 12ng/mL | 0.75 | 0.812 | 0.778 |
| 0.873 | |||
| 6ng/mL | 0.501 | 0.491 | 0.458 |
| 0.482 | |||
| 3ng/mL | 0.227 | 0.231 | 0.198 |
| 0.236 | |||
| 0ng/mL | 0.041 | 0.034 | 0 |
| 0.027 |
Precision
We measured random samples of E2051Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 28.63 | 0.97 | 3.38 |
| Intra-Assay | 2 | 18 | 14.82 | 0.84 | 5.67 |
| Intra-Assay | 3 | 18 | 8.31 | 0.57 | 6.88 |
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