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Home > Product > ELISA Kit > Mouse Endothelin-2, EDN2 ELISA Kit

Mouse Endothelin-2, EDN2 ELISA Kit

Mouse Endothelin-2, EDN2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.56ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.56ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Endothelin-2

Code

E2052Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.56ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Endothelin 2 (ET-2) is a protein encoded by the EDN2 gene in humans. It was first discovered in 1988 by Yanagisawa and team and belongs to a family of three endothelin peptide isoforms (ET-1, ET-2, ET-3), which constrict blood vessels. ET-2 is encoded by genes on separate chromosomes to its isoforms and is mainly produced in vascular endothelial cells of the kidney, placenta, uterus, heart, central nervous system and intestine. It becomes present in the blood of animals and humans at levels ranging from 0.3pg/ml to 3pg/ml. ET-2 acts by binding to two different G-protein coupled receptors (GCPRs), the endothelin A receptor (EDNRA) and the endothelin B receptor (EDNRB).

UniProt accession

MASS(Da)

19,538

GeneID

13615

Synonyms

EDN2;Endothelin-2;ET-2;PPET2;Preproendothelin-2;Vasoactive intestinal contractor;VIC

Gene names

Edn2

Research area

Cancer

Target protein

EDN2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2052Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.686 2.561 2.506
2.436
120ng/L 1.366 1.427 1.372
1.488
60ng/L 0.771 0.79 0.735
0.809
30ng/L 0.515 0.478 0.423
0.442
15ng/L 0.232 0.233 0.177
0.233
0ng/L 0.063 0.055 0
0.048

Precision

We measured random samples of E2052Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 117.16 4.31 3.68
Intra-Assay 2 18 101.18 4.62 4.56
Intra-Assay 3 18 47.62 2.56 5.38

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2052Mo

    $320.00

  • 96T

    E2052Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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