Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2112Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 2.144 | 2.101 | 2.042 |
| 2.058 | |||
| 1600ng/L | 1.666 | 1.624 | 1.565 |
| 1.582 | |||
| 800ng/L | 1.013 | 1.081 | 1.022 |
| 1.148 | |||
| 400ng/L | 0.722 | 0.719 | 0.66 |
| 0.717 | |||
| 200ng/L | 0.46 | 0.453 | 0.394 |
| 0.446 | |||
| 0ng/L | 0.062 | 0.059 | 0 |
| 0.056 |
Precision
We measured random samples of E2112Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1750.45 | 101.12 | 5.78 |
| Intra-Assay | 2 | 18 | 522.53 | 36.14 | 6.92 |
| Intra-Assay | 3 | 18 | 411.23 | 32.58 | 7.92 |
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