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Home > Product > ELISA Kit > Mouse Group 2F Secretory Phospholipase A2, PLA2G2F ELISA Kit

Mouse Group 2F Secretory Phospholipase A2, PLA2G2F ELISA Kit

Mouse Group 2F Secretory Phospholipase A2, PLA2G2F ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.227ng/mL

Detection range: 0.5-100ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.227ng/mL

Detection range: 0.5-100ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Group 2F secretory phospholipase A2

Code

E2113Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.227ng/mL

Detection range

0.5-100ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10531313). Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids (phospholipase A2 activity), the catalytic efficiency decreasing in the following order: phosphatidylglycerols > phosphatidylethanolamines > phosphatidylcholines > phosphatidylserines (PubMed:11877435, PubMed:10531313). May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases (PubMed:10531313).

UniProt accession

MASS(Da)

18,880

GeneID

26971

Synonyms

GIIF sPLA2;GIIFsPLA2;Group IIF secretory phospholipase A2;mGII;Phosphatidylcholine 2-acylhydrolase 2F;PLA2G2F;sPLA2-IIF

Gene names

Pla2g2f

Research area

Immunology

Target protein

PLA2G2F

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2113Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/mL 2.344 2.357 2.317
2.371
32ng/mL 1.354 1.253 1.212
1.153
16ng/mL 0.838 0.736 0.695
0.634
8ng/mL 0.405 0.443 0.402
0.481
4ng/mL 0.255 0.236 0.195
0.217
0ng/mL 0.033 0.041 0
0.049

Precision

We measured random samples of E2113Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.9 0.5 6.28
Intra-Assay 2 18 28.2 1.46 5.18
Intra-Assay 3 18 47.26 1.5 3.18

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2113Mo

    $320.00

  • 96T

    E2113Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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