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Home > Product > ELISA Kit > Human D-3-Phosphoglycerate Dehydrogenase, PHGDH ELISA Kit

Human D-3-Phosphoglycerate Dehydrogenase, PHGDH ELISA Kit

Human D-3-Phosphoglycerate Dehydrogenase, PHGDH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

D-3-phosphoglycerate dehydrogenase

Code

E2120Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

UniProt accession

MASS(Da)

56,651

GeneID

26227

Synonyms

2-oxoglutarate reductase;3PGDH;3-PGDH;D-3-phosphoglycerate dehydrogenase;HEL-S-113;Malate dehydrogenase;NLS;NLS1;PDG;PGAD;PGD;PGDH;PHGDH;PHGDHD

Gene names

PHGDH

Research area

Signal Transduction

Target protein

PHGDH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2120Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.03 2.159 2.089
2.288
6ng/ml 1.462 1.377 1.308
1.292
3ng/ml 0.925 0.94 0.871
0.955
1.5ng/ml 0.475 0.499 0.43
0.523
0.75ng/ml 0.22 0.244 0.175
0.269
0ng/ml 0.07 0.069 0
0.068

Precision

We measured random samples of E2120Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.14 0.2 3.98
Intra-Assay 2 18 1.15 0.07 5.98
Intra-Assay 3 18 4.45 0.3 6.75

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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