Rat Kallikrein 1, KLK 1 ELISA Kit

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Home > Product > ELISA Kit > Rat Kallikrein 1, KLK 1 ELISA Kit

Rat Kallikrein 1, KLK 1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.213ng/mL

Detection range: 0.5-100ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.213ng/mL

Detection range: 0.5-100ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Kallikrein 1

Code

E2122Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.213ng/mL

Detection range

0.5-100ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Kallikrein-1 is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

UniProt accession

P00758

MASS（Da）

28,852

GeneID

24594

Synonyms

Gamma-NGF;Kallikrein-1;KLK 1;Klk1;Klk1c1;Klna4;Nerve growth factor gamma chain;Nerve growth factor gamma chain 1;Nerve growth factor gamma chain 2];Ngfg;Pancreatic kallikrein;PS kallikrein;rGK-1;rK-1;Tissue kallikrein;True kallikrein;True tissue kallikrein

Gene names

Ngfg

Research area

Cardiovascular

Target protein

KLK 1

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E2122Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/mL	2.163	2.234	2.181
	2.305
32ng/mL	1.505	1.51	1.457
	1.515
16ng/mL	0.977	1.076	1.023
	1.175
8ng/mL	0.779	0.733	0.68
	0.687
4ng/mL	0.472	0.45	0.398
	0.429
0ng/mL	0.063	0.053	0
	0.042

Precision

We measured random samples of E2122Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.24	1.06	8.69
Intra-Assay	2	18	32.23	1.84	5.71
Intra-Assay	3	18	5.2	0.57	10.91

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E2122Ra

$320.00
96T

E2122Ra

$458.00

Add to Cart

Lead time: Within two weeks

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