Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2129Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 2.331 | 2.353 | 2.298 |
| 2.375 | |||
| 6ng/mL | 1.573 | 1.556 | 1.501 |
| 1.539 | |||
| 3ng/mL | 0.795 | 0.827 | 0.772 |
| 0.859 | |||
| 1.5ng/mL | 0.508 | 0.51 | 0.455 |
| 0.511 | |||
| 0.75ng/mL | 0.213 | 0.201 | 0.147 |
| 0.189 | |||
| 0ng/mL | 0.046 | 0.055 | 0 |
| 0.063 |
Precision
We measured random samples of E2129Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.42 | 0.07 | 4.78 |
| Intra-Assay | 2 | 18 | 3.62 | 0.22 | 6.15 |
| Intra-Assay | 3 | 18 | 6.02 | 0.24 | 4.03 |
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