Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Macrophage Colony-stimulating Factor 1 Receptor, CSF1R ELISA Kit

Rat Macrophage Colony-stimulating Factor 1 Receptor, CSF1R ELISA Kit

Rat Macrophage Colony-stimulating Factor 1 Receptor, CSF1R ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.044ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Macrophage colony-stimulating factor 1 receptor

Code

E2138Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.044ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (By similarity). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor (By similarity). In the central nervous system, may play a role in the development of microglia macrophages (By similarity).

UniProt accession

MASS(Da)

109,264

GeneID

307403

Synonyms

CD antigen CD115;c-fms;CSF-1 receptor;CSF1R;CSF-1R;CSF-1-R;Macrophage colony-stimulating factor 1 receptor;M-CSF-R;mrfms;Proto-oncogene c-Fms

Gene names

Csf1r

Research area

Cardiovascular

Target protein

CSF1R

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2138Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.281 2.234 2.183
2.188
12ng/ml 1.513 1.41 1.358
1.306
6ng/ml 0.715 0.702 0.651
0.69
3ng/ml 0.35 0.342 0.29
0.334
1.5ng/ml 0.161 0.172 0.12
0.182
0ng/ml 0.046 0.052 0
0.058

Precision

We measured random samples of E2138Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.47 0.16 3.55
Intra-Assay 2 18 9.14 0.4 4.41
Intra-Assay 3 18 11.49 0.29 2.53

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E2138Ra

    $320.00

  • 96T

    E2138Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up