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Home > Product > ELISA Kit > Rat Matrix Metallopeptidase 8, Neutrophil Collagenase, MMP8 ELISA Kit

Rat Matrix Metallopeptidase 8, Neutrophil Collagenase, MMP8 ELISA Kit

Rat Matrix Metallopeptidase 8, Neutrophil Collagenase, MMP8 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/mL

Detection range: 0.1-40ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

matrix metallopeptidase 8,neutrophil collagenase

Code

E2143Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/mL

Detection range

0.1-40ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Neutrophil collagenase, also known as matrix metalloproteinase-8 (MMP-8) or PMNL collagenase (MNL-CL), is a collagen cleaving enzyme which is present in the connective tissue of most mammals. In humans, the MMP-8 protein is encoded by the MMP8 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage.

UniProt accession

MASS(Da)

53,277

GeneID

63849

Synonyms

Matrix Metalloproteinase-8;MMP8;MMP-8;Neutrophil collagenase

Gene names

Mmp8

Research area

Cardiovascular

Target protein

MMP8

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2143Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/mL 2.14 2.17 2.127
2.199
12ng/mL 1.503 1.42 1.377
1.337
6ng/mL 0.83 0.864 0.821
0.898
3ng/mL 0.397 0.428 0.385
0.459
1.5ng/mL 0.278 0.289 0.246
0.299
0ng/mL 0.04 0.043 0
0.045

Precision

We measured random samples of E2143Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.24 0.13 5.73
Intra-Assay 2 18 10.35 0.38 3.71
Intra-Assay 3 18 5.88 0.26 4.47

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2143Ra

    $320.00

  • 96T

    E2143Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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