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Home > Product > ELISA Kit > Rat Muscarinic Acetylcholine Receptor M3, CHRM3 ELISA Kit

Rat Muscarinic Acetylcholine Receptor M3, CHRM3 ELISA Kit

Rat Muscarinic Acetylcholine Receptor M3, CHRM3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.033ng/mL

Detection range: 0.06-18ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.033ng/mL

Detection range: 0.06-18ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Muscarinic acetylcholine receptor M3

Code

E2158Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.033ng/mL

Detection range

0.06-18ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

UniProt accession

MASS(Da)

66,066

GeneID

24260

Synonyms

CHRM3;Muscarinic acetylcholine receptor M3

Gene names

Chrm3

Research area

Neuroscience

Target protein

CHRM3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2158Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
9.6ng/mL 2.18 2.184 2.153
2.188
4.8ng/mL 1.182 1.32 1.289
1.459
2.4ng/mL 0.765 0.728 0.696
0.69
1.2ng/mL 0.389 0.41 0.378
0.431
0.6ng/mL 0.147 0.169 0.138
0.191
0ng/mL 0.029 0.031 0
0.034

Precision

We measured random samples of E2158Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.59 0.26 4.01
Intra-Assay 2 18 2.1 0.11 5.23
Intra-Assay 3 18 3.91 0.19 4.75

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2158Ra

    $320.00

  • 96T

    E2158Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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