Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2161Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/L | 2.12 | 2.238 | 2.183 |
| 2.357 | |||
| 20ng/L | 1.615 | 1.647 | 1.592 |
| 1.679 | |||
| 10ng/L | 1.183 | 1.145 | 1.09 |
| 1.107 | |||
| 5ng/L | 0.78 | 0.744 | 0.689 |
| 0.708 | |||
| 2.5ng/L | 0.445 | 0.449 | 0.393 |
| 0.453 | |||
| 0ng/L | 0.064 | 0.055 | 0 |
| 0.047 |
Precision
We measured random samples of E2161Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 7.82 | 0.52 | 6.62 |
| Intra-Assay | 2 | 18 | 13.15 | 0.69 | 5.26 |
| Intra-Assay | 3 | 18 | 2.59 | 0.21 | 8.09 |
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