Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2165Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/L | 1.966 | 1.987 | 1.947 |
| 2.008 | |||
| 40ng/L | 1.415 | 1.406 | 1.366 |
| 1.398 | |||
| 20ng/L | 0.748 | 0.752 | 0.712 |
| 0.756 | |||
| 10ng/L | 0.352 | 0.333 | 0.293 |
| 0.315 | |||
| 5ng/L | 0.166 | 0.19 | 0.15 |
| 0.214 | |||
| 0ng/L | 0.058 | 0.04 | 0 |
| 0.023 |
Precision
We measured random samples of E2165Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 32.02 | 1.68 | 5.26 |
| Intra-Assay | 2 | 18 | 38.32 | 1.39 | 3.62 |
| Intra-Assay | 3 | 18 | 16.03 | 0.71 | 4.44 |
Protocols
References
Customer reviews and Q&As
