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Home > Product > ELISA Kit > Mouse Isocitrate Dehydrogenase, Nad Subunit Alpha, Mitochondrial, IDH3A ELISA Kit

Mouse Isocitrate Dehydrogenase, Nad Subunit Alpha, Mitochondrial, IDH3A ELISA Kit

Mouse Isocitrate Dehydrogenase, Nad Subunit Alpha, Mitochondrial, IDH3A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.27ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.27ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Isocitrate dehydrogenase,NAD subunit alpha, mitochondrial

Code

E2194Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.27ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

UniProt accession

MASS(Da)

39,639

GeneID

67834

Synonyms

1110003P10Rik;1500012E04Rik;AA407078;AI316514;IDH3A;Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial;Isocitric dehydrogenase subunit alpha;NAD(+-specific ICDH subunit alpha

Gene names

Idh3a

Research area

Others

Target protein

IDH3A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2194Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.337 2.242 2.18
2.146
600ng/L 1.445 1.369 1.308
1.294
300ng/L 0.734 0.8 0.738
0.865
150ng/L 0.535 0.495 0.433
0.455
75ng/L 0.319 0.3 0.238
0.281
0ng/L 0.065 0.062 0
0.058

Precision

We measured random samples of E2194Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 644.46 24.09 3.74
Intra-Assay 2 18 428.81 23.19 5.41
Intra-Assay 3 18 204.36 11.47 5.62

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2194Mo

    $320.00

  • 96T

    E2194Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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