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Home > Product > ELISA Kit > Mouse Klotho, KL ELISA Kit

Mouse Klotho, KL ELISA Kit

Mouse Klotho, KL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.58ng/L

Detection range: 1-400ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Klotho

Code

E2201Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.58ng/L

Detection range

1-400ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

UniProt accession

MASS(Da)

116,398

GeneID

16591

Synonyms

alp;alpha-kl;KL;Klotho;Klotho Peptide]

Gene names

Kl

Research area

Others

Target protein

KL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2201Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
240ng/L 2.65 2.539 2.487
2.429
120ng/L 1.343 1.435 1.383
1.526
60ng/L 0.847 0.78 0.728
0.713
30ng/L 0.464 0.49 0.438
0.516
15ng/L 0.285 0.284 0.232
0.283
0ng/L 0.039 0.052 0
0.066

Precision

We measured random samples of E2201Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 39.02 2.77 7.1
Intra-Assay 2 18 66.8 3.23 4.83
Intra-Assay 3 18 127.82 4.18 3.27

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2201Mo

    $320.00

  • 96T

    E2201Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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