Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2204Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.27 | 2.3 | 2.251 |
| 2.329 | |||
| 600ng/L | 1.214 | 1.185 | 1.136 |
| 1.156 | |||
| 300ng/L | 0.733 | 0.73 | 0.681 |
| 0.728 | |||
| 150ng/L | 0.396 | 0.425 | 0.376 |
| 0.454 | |||
| 75ng/L | 0.261 | 0.229 | 0.179 |
| 0.196 | |||
| 0ng/L | 0.051 | 0.049 | 0 |
| 0.047 |
Precision
We measured random samples of E2204Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 556.21 | 32.6 | 5.86 |
| Intra-Assay | 2 | 18 | 822.8 | 25.14 | 3.05 |
| Intra-Assay | 3 | 18 | 296.81 | 18.64 | 6.28 |
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