Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2207Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.565 | 2.581 | 2.537 |
| 2.597 | |||
| 1200ng/L | 1.458 | 1.39 | 1.346 |
| 1.323 | |||
| 600ng/L | 0.857 | 0.814 | 0.77 |
| 0.772 | |||
| 300ng/L | 0.44 | 0.417 | 0.373 |
| 0.395 | |||
| 150ng/L | 0.26 | 0.262 | 0.217 |
| 0.264 | |||
| 0ng/L | 0.037 | 0.044 | 0 |
| 0.052 |
Precision
We measured random samples of E2207Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1154.58 | 38.41 | 3.33 |
| Intra-Assay | 2 | 18 | 442.76 | 24.77 | 5.59 |
| Intra-Assay | 3 | 18 | 892.47 | 43.8 | 4.91 |
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