Full product name
Leucine-rich repeat serine/threonine-protein kinase 2
Code
E2208Mo
Assay type
Sandwich
Size
48T,96T
Sensitivity
9.09ng/L
Detection range
20-4500ng/L
Sample type
Serum, plasma, cell culture supernates
Species
Mouse
Storage
2-8ºC
Assay time
1h 30m
Background
Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, autophagy, and vesicle trafficking (PubMed:26824392, PubMed:29125462, PubMed:28720718, PubMed:30398148, PubMed:29212815).
Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation (PubMed:26824392, PubMed:28720718, PubMed:30398148, PubMed:29212815, PubMed:29125462).
Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43 (PubMed:26824392, PubMed:28720718, PubMed:30398148, PubMed:29212815).
Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A (By similarity).
Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A (By similarity).
Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain (PubMed:29125462, PubMed:30398148).
Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (By similarity).
Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity).
Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization (PubMed:25201882).
Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway (By similarity).
The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes (By similarity).
Phosphorylates PRDX3 (By similarity).
By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis (PubMed:28720718).
Independent of its kinase activity, inhibits the proteosomal degradation of MAPT, thus promoting MAPT oligomerization and secretion (By similarity).
In addition, has GTPase activity via its Roc domain which regulates LRKK2 kinase activity (By similarity).
UniProt accession
MASS（Da）
284,732
GeneID
66725
Synonyms
4921513O20Rik;9330188B09Rik;AW561911;cI-4;cI-46;Gm927;Leucine-rich repeat serine/threonine-protein kinase 2;LRRK2
Gene names
Lrrk2
Research area
Others
Target protein
LRRK2
