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Home > Product > ELISA Kit > Rat Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, PLOD1 ELISA Kit

Rat Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, PLOD1 ELISA Kit

Rat Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, PLOD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Code

E2209Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:7578263). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (By similarity).

UniProt accession

MASS(Da)

83,612

GeneID

116552

Synonyms

LH1;Lysyl hydroxylase 1;Plod;PLOD1;Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene names

Plod1

Research area

Others

Target protein

PLOD1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2209Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 2.051 2.049 2.013
2.047
6ng/mL 0.999 1.041 1.006
1.084
3ng/mL 0.591 0.502 0.466
0.413
1.5ng/mL 0.217 0.244 0.208
0.271
0.75ng/mL 0.139 0.122 0.086
0.105
0ng/mL 0.033 0.035 0
0.038

Precision

We measured random samples of E2209Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.17 0.22 4.17
Intra-Assay 2 18 7.6 0.2 2.57
Intra-Assay 3 18 2.53 0.11 4.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2209Ra

    $320.00

  • 96T

    E2209Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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