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Home > Product > ELISA Kit > Mouse Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, ACADL ELISA Kit

Mouse Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, ACADL ELISA Kit

Mouse Long-chain Specific Acyl-CoA Dehydrogenase, Mitochondrial, ACADL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.56ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.56ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Long-chain specific acyl-CoA dehydrogenase, mitochondrial

Code

E2217Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.56ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed:9861014).

UniProt accession

MASS(Da)

47,908

GeneID

11363

Synonyms

AA960361;ACADL;AU018452;C79855;LC;LCAD;Long-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene names

Acadl

Research area

Others

Target protein

ACADL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2217Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.424 2.313 2.295
2.202
240ng/L 1.194 1.252 1.234
1.31
120ng/L 0.563 0.566 0.549
0.57
60ng/L 0.394 0.416 0.398
0.438
30ng/L 0.185 0.152 0.134
0.118
0ng/L 0.017 0.018 0
0.018

Precision

We measured random samples of E2217Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 177.21 8.27 4.67
Intra-Assay 2 18 291.85 9.92 3.4
Intra-Assay 3 18 124.82 8.3 6.65

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2217Mo

    $320.00

  • 96T

    E2217Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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