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Home > Product > ELISA Kit > Rat Receptor Tyrosine-protein Kinase Erbb-4, ERBB4 ELISA Kit

Rat Receptor Tyrosine-protein Kinase Erbb-4, ERBB4 ELISA Kit

Rat Receptor Tyrosine-protein Kinase Erbb-4, ERBB4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 33.86ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 33.86ng/L

Detection range: 70-15000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Receptor tyrosine-protein kinase erbB-4

Code

E2231Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

33.86ng/L

Detection range

70-15000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

UniProt accession

MASS(Da)

146,958

GeneID

59323

Synonyms

4ICD;E4ICD;ERBB4;ERBB4 intracellular domain;Proto-oncogene-like protein c-ErbB-4;Receptor tyrosine-protein kinase erbB-4;s80HER4]

Gene names

Erbb4

Research area

Signal Transduction

Target protein

ERBB4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2231Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8000ng/L 2.142 2.131 2.106
2.121
4000ng/L 1.217 1.314 1.289
1.411
2000ng/L 0.766 0.741 0.716
0.716
1000ng/L 0.34 0.35 0.325
0.361
500ng/L 0.171 0.17 0.144
0.168
0ng/L 0.028 0.025 0
0.022

Precision

We measured random samples of E2231Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4059.15 124.49 3.07
Intra-Assay 2 18 2677.67 133.72 4.99
Intra-Assay 3 18 1471.4 50.54 3.43

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2231Ra

    $320.00

  • 96T

    E2231Ra

    $458.00

Add to Cart

Lead time: Within two weeks

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