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Home > Product > ELISA Kit > Mouse Mesoderm-specific Transcript Homolog Protein, MEST ELISA Kit

Mouse Mesoderm-specific Transcript Homolog Protein, MEST ELISA Kit

Mouse Mesoderm-specific Transcript Homolog Protein, MEST ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.34ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.34ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Mesoderm-specific transcript homolog protein

Code

E2247Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.34ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Mesoderm-specific transcript homolog protein is a protein that in humans is encoded by the MEST gene. This gene encodes a member of the Alpha/beta hydrolase superfamily and has isoform-specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promoter switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6.

UniProt accession

MASS(Da)

38,907

GeneID

17294

Synonyms

AA408879;AI256745;Mesoderm-specific transcript protein;MEST;Paternally-expressed gene 1 protein;Peg;Peg1

Gene names

Mest

Research area

Others

Target protein

MEST

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2247Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.556 2.564 2.521
2.572
1200ng/L 1.578 1.475 1.432
1.373
600ng/L 0.802 0.792 0.75
0.783
300ng/L 0.474 0.476 0.433
0.478
150ng/L 0.217 0.227 0.184
0.236
0ng/L 0.042 0.043 0
0.044

Precision

We measured random samples of E2247Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 682.23 43.52 6.38
Intra-Assay 2 18 1130.67 31.3 2.77
Intra-Assay 3 18 412.07 24.63 5.98

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2247Mo

    $320.00

  • 96T

    E2247Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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