Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2248Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/mL | 2.212 | 2.285 | 2.23 |
| 2.358 | |||
| 8ng/mL | 1.375 | 1.392 | 1.337 |
| 1.409 | |||
| 4ng/mL | 0.904 | 0.832 | 0.777 |
| 0.76 | |||
| 2ng/mL | 0.488 | 0.462 | 0.407 |
| 0.436 | |||
| 1ng/mL | 0.262 | 0.288 | 0.233 |
| 0.314 | |||
| 0ng/mL | 0.039 | 0.055 | 0 |
| 0.071 |
Precision
We measured random samples of E2248Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 7.22 | 0.2 | 2.8 |
| Intra-Assay | 2 | 18 | 2.29 | 0.13 | 5.48 |
| Intra-Assay | 3 | 18 | 5.82 | 0.36 | 6.13 |
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