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Home > Product > ELISA Kit > Mouse Mitotic Checkpoint Serine Threonine-protein Kinase Bub1 Beta, BUB1B ELISA Kit

Mouse Mitotic Checkpoint Serine Threonine-protein Kinase Bub1 Beta, BUB1B ELISA Kit

Mouse Mitotic Checkpoint Serine Threonine-protein Kinase Bub1 Beta, BUB1B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.08ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.08ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Code

E2265Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.08ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Essential component of the mitotic checkpoint. Required for normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility (By similarity).

UniProt accession

MASS(Da)

118,392

GeneID

12236

Synonyms

AU045529;BUB1B;BUBR;BUBR1;MAD3/BUB1-related protein kinase;Mitotic checkpoint kinase MAD3L;Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene names

Bub1b

Research area

Cell Biology

Target protein

BUB1B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2265Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.153 2.218 2.165
2.284
1200ng/L 1.743 1.687 1.633
1.63
600ng/L 1.13 1.08 1.027
1.03
300ng/L 0.755 0.71 0.657
0.665
150ng/L 0.428 0.414 0.36
0.4
0ng/L 0.052 0.053 0
0.055

Precision

We measured random samples of E2265Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 330.6 24.94 7.55
Intra-Assay 2 18 776.44 24.5 3.16
Intra-Assay 3 18 498 34.1 6.85

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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