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Home > Product > ELISA Kit > Human Carbonic Anhydrase 5B, Mitochondrial, CA5B ELISA Kit

Human Carbonic Anhydrase 5B, Mitochondrial, CA5B ELISA Kit

Human Carbonic Anhydrase 5B, Mitochondrial, CA5B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.17ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.17ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carbonic Anhydrase 5B,Mitochondrial

Code

E2271Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.17ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Carbonic anhydrase 5B, mitochondrial is an enzyme that in humans is encoded by the CA5B gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.

UniProt accession

MASS(Da)

36,434

GeneID

11238

Synonyms

CA5B;Carbonate dehydratase VB;Carbonic anhydrase 5B, mitochondrial;Carbonic anhydrase VB;CAVB;CA-VB

Gene names

CA5B

Research area

Tags & Cell Markers

Target protein

CA5B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Carbonic Anhydrase 5B, Mitochondrial, CA5B ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.398 2.292 2.251
2.187
800ng/L 1.207 1.215 1.174
1.224
400ng/L 0.64 0.642 0.601
0.645
200ng/L 0.381 0.357 0.316
0.333
100ng/L 0.206 0.161 0.12
0.117
0ng/L 0.044 0.041 0
0.038

Precision

We measured random samples of E2271Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 686.95 35.53 5.17
Intra-Assay 2 18 1109.29 36.22 3.27
Intra-Assay 3 18 408.66 21.33 5.22

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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