Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2275Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.382 | 2.371 | 2.338 |
| 2.36 | |||
| 1200ng/L | 1.448 | 1.381 | 1.348 |
| 1.313 | |||
| 600ng/L | 0.727 | 0.651 | 0.617 |
| 0.575 | |||
| 300ng/L | 0.326 | 0.34 | 0.307 |
| 0.354 | |||
| 150ng/L | 0.125 | 0.158 | 0.125 |
| 0.191 | |||
| 0ng/L | 0.018 | 0.033 | 0 |
| 0.048 |
Precision
We measured random samples of E2275Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1615.98 | 46.09 | 2.85 |
| Intra-Assay | 2 | 18 | 1001.37 | 43.06 | 4.3 |
| Intra-Assay | 3 | 18 | 407.08 | 21.4 | 5.26 |
Protocols
References
Customer reviews and Q&As
Associated products
