Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
N/A
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2279Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 400ng/ml | 2.211 | 2.259 | 2.212 |
| 2.306 | |||
| 200ng/ml | 1.736 | 1.737 | 1.69 |
| 1.738 | |||
| 100ng/ml | 1.246 | 1.249 | 1.202 |
| 1.251 | |||
| 50ng/ml | 0.618 | 0.679 | 0.632 |
| 0.74 | |||
| 25ng/ml | 0.368 | 0.389 | 0.342 |
| 0.409 | |||
| 0ng/ml | 0.062 | 0.047 | 0 |
| 0.032 |
Precision
We measured random samples of E2279Bo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 193.92 | 12.83 | 6.62 |
| Intra-Assay | 2 | 18 | 92.78 | 5.34 | 5.75 |
| Intra-Assay | 3 | 18 | 33.36 | 1.77 | 5.29 |
Protocols
References
Customer reviews and Q&As
