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Home > Product > ELISA Kit > Mouse Myosin Light Chain Kinase, Smooth Muscle, MYLK ELISA Kit

Mouse Myosin Light Chain Kinase, Smooth Muscle, MYLK ELISA Kit

Mouse Myosin Light Chain Kinase, Smooth Muscle, MYLK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.41ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 5.41ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Myosin light chain kinase, smooth muscle

Code

E2281Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.41ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.

UniProt accession

MASS(Da)

86,372

GeneID

213435

Synonyms

Cardiac-MLCK;Cardiac-MyBP-C-associated Ca/CaM kinase;D830007F02Rik;MLCK;MYLK;Myosin light chain kinase 3

Gene names

Mylk3

Research area

Others

Target protein

MYLK

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2281Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.119 2.18 2.122
2.241
600ng/L 1.174 1.187 1.129
1.2
300ng/L 0.774 0.774 0.715
0.774
150ng/L 0.455 0.443 0.384
0.43
75ng/L 0.214 0.208 0.149
0.201
0ng/L 0.064 0.059 0
0.054

Precision

We measured random samples of E2281Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 494.55 24.7 5
Intra-Assay 2 18 678.78 24.11 3.55
Intra-Assay 3 18 208.73 12.51 5.99

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2281Mo

    $320.00

  • 96T

    E2281Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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