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Home > Product > ELISA Kit > Human Claudin 15, CLDN15 ELISA Kit

Human Claudin 15, CLDN15 ELISA Kit

Human Claudin 15, CLDN15 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.15ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Claudin 15

Code

E2311Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.15ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN15 forms tight junctions that mediate the paracellular transport of small monovalent cations along a concentration gradient, due to selective permeability for Na+, Li+ and K+ ions, but selects against Cl- ions. Plays an important role in paracellular Na+ transport in the intestine and in Na+ homeostasis. Required for normal Na+-dependent intestinal nutrient uptake.

UniProt accession

MASS(Da)

24,356

GeneID

24146

Synonyms

Claudin-15;CLDN15

Gene names

CLDN15

Research area

Signal Transduction

Target protein

CLDN15

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2311Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.039 2.183 2.141
2.327
600ng/L 1.745 1.639 1.597
1.534
300ng/L 1.009 1.04 0.998
1.071
150ng/L 0.726 0.733 0.691
0.741
75ng/L 0.415 0.435 0.392
0.455
0ng/L 0.047 0.043 0
0.039

Precision

We measured random samples of E2311Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 284.27 19.62 6.9
Intra-Assay 2 18 752.13 33.46 4.45
Intra-Assay 3 18 147.16 12.26 8.33

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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