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Home > Product > ELISA Kit > Rat Ubia Prenyltransferase Domain-containing Protein 1, UBIAD1 ELISA Kit

Rat Ubia Prenyltransferase Domain-containing Protein 1, UBIAD1 ELISA Kit

Rat Ubia Prenyltransferase Domain-containing Protein 1, UBIAD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.36ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 9.36ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

UbiA prenyltransferase domain-containing protein 1

Code

E2311Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.36ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosynthetic enzyme: coenzyme Q10, also named ubiquinone, plays an important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress (By similarity).

UniProt accession

MASS(Da)

37,026

GeneID

313706

Synonyms

RGD1309588;UbiA prenyltransferase domain-containing protein 1;UBIAD1

Gene names

Ubiad1

Research area

Others

Target protein

UBIAD1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2311Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.324 2.227 2.184
2.129
1200ng/L 1.169 1.262 1.22
1.355
600ng/L 0.627 0.675 0.633
0.724
300ng/L 0.409 0.438 0.396
0.467
150ng/L 0.198 0.232 0.19
0.267
0ng/L 0.054 0.042 0
0.031

Precision

We measured random samples of E2311Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1901.95 43.43 2.28
Intra-Assay 2 18 921.78 44.51 4.83
Intra-Assay 3 18 461.88 31.7 6.86

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2311Ra

    $320.00

  • 96T

    E2311Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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