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Home > Product > ELISA Kit > Mouse Phenylalanine-4-hydroxylase, PAH ELISA Kit

Mouse Phenylalanine-4-hydroxylase, PAH ELISA Kit

Mouse Phenylalanine-4-hydroxylase, PAH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.92ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 7.92ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Phenylalanine-4-hydroxylase

Code

E2339Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.92ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Phenylalanine hydroxylase (PAH) (EC 1.14.16.1) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. PAH is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. During the reaction, molecular oxygen is heterolytically cleaved with sequential incorporation of one oxygen atom into BH4 and phenylalanine substrate. In humans, mutations in its encoding gene, PAH, can lead to the metabolic disorder phenylketonuria.

UniProt accession

MASS(Da)

51,900

GeneID

18478

Synonyms

AW106920;PAH;Phe-4-monooxygenase;Phenylalanine-4-hydroxylase

Gene names

Pah

Research area

Signal Transduction

Target protein

PAH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2339Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.252 2.278 2.232
2.305
800ng/L 1.319 1.319 1.273
1.319
400ng/L 0.798 0.796 0.75
0.793
200ng/L 0.51 0.47 0.424
0.43
100ng/L 0.208 0.209 0.163
0.21
0ng/L 0.059 0.046 0
0.033

Precision

We measured random samples of E2339Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 387.58 21.29 5.49
Intra-Assay 2 18 808.43 26.8 3.32
Intra-Assay 3 18 319.63 21.46 6.71

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2339Mo

    $320.00

  • 96T

    E2339Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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